Vector Core Services
How to order:
For
your convenience, the UNC Vector Core Order Form is available online.
Please email completed form to Lori Nisi.
March 2013 - We are implementing a new billing system, and the following changes apply:
- We must receive a PO# or indication of credit card payment on your order form before your order can be entered in our system.
- For credit card orders, you will now receive an emailed secure link to enter the credit card payment after your order ships (instead of providing the information over the phone).
- Within the next month, we will transition to an online ordering system. This will replace the Vector Core order form submission process. An email will be sent out before the transition.
If you have any questions regarding the ordering or billing process, please contact Lori Nisi. For questions regarding vector availability or vector production, please contact Xinghua Zeng via email or phone at 919.537.3100.
*Please note: The Vector Core (AAV and Adenovirus) has relocated to:
6101 Quadrangle Dr..
Room 206
Chapel Hill, NC 27517
Ph: 919.537.3100
*The lentiviral lab has not moved, and will remain at the address listed under lentivirus ordering instructions.
Available Services:
* Please click here for vector storage and handling information
* Please click here for our in-stock virus inventory
How to pay:
External customers: We accept POs and Visa/Mastercard.
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PO payment - Please write PO# on order form. An invoice will be sent to the accounting contact after order is shipped.
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Visa/MC - Please indicate you would like to pay with a card on the order form and provide a contact for the card. You will receive an email with a secure link to enter your credit card information. We do not charge until your order has been shipped.
- Shipping Fee: Vectors are shipped via Fedex. Shipping charges are $40 per domestic order, $150 per international.
UNC customers: Please provide a funding account number.
AAV Vector Production (serotypes 1-6, 2.5, 8, 9) AAV MSDS
Our new AAV vector production method utilizes a triple transfection protocol in suspension HEK293 cells and is purified by column chromotography. This system is serum free, antibiotic free, generates less empty particles, has higher purity and is scalable to Wave bioreactors. We offer custom vector production and aliquots of in-stock vectors.
Custom AAV Vector Production
For custom vector preps, please send at least 300ug of plasmid. Approximate turnaround time is 2-3 weeks. We guarantee a titer of 10E11, although average yield is usually ~10E12. Yield is dependent on customer construct.
Prices per Custom Prep:
External Academic: $2,200
UNC Investigator: $1,500
Corporate: $3,400
Please send plasmids via FedEx or UPS to:
UNC Vector Core
Attn: Xinghua Zeng
6101 Quadrangle Dr.
Room 206
Chapel Hill, NC 27517
*Please include a copy of your order form and a Sma1 restriction map with your plasmid shipment.
In-Stock AAV Vectors, 100ul Aliquots
Prices per 100ul aliquot:
External Academic: $220
UNC Investigator: $150
Corporate: $340
The following viruses are available in AAV1, 2, 2.5, 5, 6, 8 & 9:
- GFP (CMV and CBA promoters)
- Self-complementary GFP (CMV, CBA, CBh promoters)
- AAV-CMV-Luciferase
- AAV-CMV-lacZ
In addition to our reporter gene inventory, we are pleased to stock optogenetic constructs from Dr. Karl Deisseroth at Stanford University, Dr. Ed Boyden at MIT, Dr. Bryan Roth at UNC and Dr. Naoshige Uchida at Harvard.
Dr. Karl Deisseroth Constructs
For first time orders of these viruses, Laboratory PIs please contact Dr. Karl Deisseroth and Charu Ramakrishnan for an MTA. Please submit a copy of your MTA along with your Vector Core order form each time you place an order for these vectors.
The following constructs are available in AAV2 and AAV5. For other serotypes, please place an order for a custom prep.
Optical Excitation: ChR2
- AAV-CaMKIIa-hChR2(H134R)-EYFP
- AAV-CaMKIIa-hChR2(H134R)-mCherry
- AAV-hSyn-hChR2(H134R)-EYFP
- AAV-hSyn-hChR2(H134R)-mCherry
Cre-inducible AAV: DIO-AAV
- AAV-EF1a-DIO-hChR2(H134R)-EYFP
- AAV-EF1a-DIO-hChR2(H134R)-mCherry
Third-generation Optogenetic Inhibition: eNpHR3.0
- AAV-EF1a-DIO-eNpHR3.0-EYFP
- AAV-EF1a-DIO-eNpHR3.0-mCherry
- AAV-CaMKIIa-eNpHR3.0-EYFP
- AAV-CaMKIIa-eNpHR3.0-mCherry
- AAV-hSyn-eNpHR3.0-EYFP
- AAV-hSyn-eNpHR3.0-mCherry
- AAV-hThy1-eNpHR3.0-EYFP
Red-shifted Optical Excitation: C1V1 Variants
- AAV-CaMKIIa-C1V1(E122T/E162T)-TS-EYFP
- AAV-CaMKIIa-C1V1(E122T/E162T)-TS-mCherry
- AAV-EF1a-DIO-C1V1(E122T/E162T)-TS-EYFP
- AAV-EF1a-DIO-C1V1(E122T/E162T)-TS-mCherry
Stabilized Step Function Opsins: SSFOs
- AAV-CaMKIIa-hChR2(C128S/D156A)-EYFP
- AAV-CaMKIIa-hChR2(C128S/D156A)-mCherry
- AAV-EF1a-DIO-hChR2(C128S/D156A)-EYFP
- AAV-EF1a-DIO-hChR2(C128S/D156A)-mCherry
Second-generation Ultrafast Optogenetic Control
- AAV-CaMKIIa-hChR2(E123T/T159C)-EYFP
- AAV-CaMKIIa-hChR2(E123T/T159C)-mCherry
- AAV-CaMKII-hChR2(E123A)-EYFP
- AAV-CaMKII-hChR2(E123A)-mCherry
- AAV-EF1a-DIO-hChR2(E123T/T159C)-EYFP
- AAV-EF1a-DIO-hChR2(E123T/T159C)-mCherry
- AAV-EF1a-DIO-ChR2(T159C)-EYFP
- AAV-EF1a-DIO-ChR2(T159C)-mCherry
- AAV-EF1a-DIO-ChR2(E123A)-EYFP
- AAV-EF1a-DIO-ChR2(E123A)-mCherry
- AAV-hSyn-ChR2(E123A)-EYFP
Third-generation Optogenetic Inhibition: Arch 3.0 & ArchT 3.0
- AAV-CaMKIIa-eArch3.0-EYFP
- AAV-hSyn-eArch3.0-EYFP
- AAV-EF1a-DIO-eArch3.0-EYFP
- AAV-CaMKIIa-eArchT3.0-EYFP
Transsynaptic Tracers: WGA-Cre
- AAV-EF1a-mCherry-IRES-WGA-Cre
Ultrafast Optogenetic Control: ChETA
Control Fluorophores
- AAV-CaMKIIa-EYFP
- AAV-CaMKIIa-mCherry
- AAV-EF1a-DIO-EYFP
- AAV-EF1a-DIO-mCherry
- AAV-hSyn-EYFP
- AAV-hSyn-mCherry
Please contact Xinghua Zeng to inquire about available serotypes for the below GFAP constructs:
- AAV-GFAP-hChR2(H134R)-EYFP
- AAV-GFAP-hChR2(H134R)-mCherry
Dr. Ed Boyden Constructs
The following constructs are available in serotypes 1, 2, 5, 8 & 9. For other serotypes, please place an order for a custom prep. No MTA is required.
Neural Silencers
- AAV-CAG-ArchT-GFP
- AAV-CAG-FLEX-ArchT-GFP
Neural Activators
The following constructs are available in serotypes 2 & 5:
Neural Silencers
- AAV-CAG-FLEX-Arch-GFP
- AAV-CAG-FLEX-ArchT-tdTomato
- AAV-CAG-Arch-GFP
- AAV-CAG-ArchT-tdTomato
- AAV-CaMKII-ArchT-GFP
Control Vectors
- AAV-CAG-FLEX-GFP
- AAV-CAG-GFP
- AAV-CAG-FLEX-tdTomato
- AAV-CAG-tdTomato
Dr. Bryan Roth Constructs
The following constructs are available in AAV2, 5 & 8. For other serotypes, please place an order for a custom prep. No MTA is required.
Cre Dependent DREADDs
- AAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine
- AAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine
- AAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine
- AAV-hSyn-DIO-EGFP
- AAV-hSyn-DIO-hM3D(Gq)-mCherry
- AAV-hSyn-DIO-hM4D(Gi)-mCherry
- AAV-hSyn-DIO-rM3D(Gs)-mCherry
- AAV-hSyn-DIO-mCherry
- AAV-EF1a-DIO-hM3D(Gq)-mCherry
- AAV-EF1a-DIO-hM4D(Gi)-mCherry
- AAV-EF1a-DIO-mCherry
Non-Cre Dependent DREADDs
- AAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine
- AAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine
- AAV-hSyn-EGFP
- AAV-CaMKIIa-HA-hM3D(Gq)-IRES-mCitrine
- AAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine
- AAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine
- AAV-CaMKIIa-EGFP
- AAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine
- AAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine
- AAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine
- AAV-GFAP-EGFP
Dr. Naoshige Uchida Constructs
The following constructs are available in AAV1, 5 & 8. For other serotypes, please place an order for a custom prep. No MTA is required.
- AAV-CA-FLEX-RG
- AAV-EF1a-FLEX-TVAmCherry
For large scale, preclinical or clinical AAV vector production, please contact David Dismuke.
Adenovirus Amplification Prep Adenovirus MSDS
Recombinant Ad vectors are amplified in 293 cells and purified with 2 consecutive CsCl gradients. Vectors are titered by infecting target cells and detecting infected cells with antibody staining. Preps have a guaranteed titer of 10E12 total particles and approximate turnaround time is 3-4 weeks.
Prices per 1ml Prep:
External Academic: $900
UNC Investigator: $600
Corporate: $1400
In-Stock Adenovirus Vectors, 100ul Aliquots
Prices per 100ul aliquot:
External Academic: $90
UNC Investigator: $60
Corporate: $140
Generation of Adenovirus Recombinants Adenovirus MSDS
The AdEasy system as described by Vogelstein's laboratory is used to incorporate new genes into Ad vectors. Cell lysates are then provided to the investigator for testing prior to amplification of the vector. Approximate turnaround time is 6 weeks.
Prices:
External Acadmic: $1,400
UNC Investigator: $900
Corporate: $2,200
Lentivirus Vectors Lentivirus MSDS
Recombinant lentiviral vectors are produced in 293T cells by transfection and can be purified/concentrated using sucrose gradients or by size exclusion. Titers of physical vector particles are determined by p24 gag ELISA. We translate 1 ng of p24 to 10E4 IU (for most vectors this is a significant underestimation of the actual vector titer). Safety assays for replication competant retroviruses (RCR) include marker rescue assay and gag and tat transfer assays. For information on lentivirus production, please contact Dr. Tal Kafri at 919-843-7365.
High Titer Concentrated/Purified Lentivirus Preps (100ul, >10E9 iu/ml)
For in vivo gene delivery studies, the UNC Vector Core recommends using high titer concentrated/purified vectors. Approximate turnaround time is 6-7 weeks (this includes mandatory safety assays to rule out the emergence of replication competent retrovirus). Please send at least 350ug of plasmid concentrated at 0.5 - 1.5mg/ml extracted by either Midi/Maxi prep columns or by cesium gradient. Alternatively, plasmid DNA can be prepared by the Vector Core for $250.
Please send plasmids to:
Dr. Tal Kafri
UNC Gene Therapy Center
5019 Thurston Bowles
Chapel Hill, NC 27599
Prices per Concentrated Prep (*includes RCR assay):
External Academic: $2,350
UNC Investigator: $1,550
Corporate: $3,050
High Titer Non-Purified Lentivirus Custom Prep (100ul, 10E8-5x10E9 IU/ml)
Vectors in this category are suitable for in vitro studies which require gene delivery to relatively non-permissive target cells. Typical vector titers range between 10E9 - 5x10E9 IU/ml (determined by the nature of the vector cassette). The vectors are concentrated by size exclusion (RCR assays are not mandatory). A total prep of 100ul can be provided in aliquots of either 10, 20 or 100ul. Approximate turnaround time is 2-3 weeks.
Prices per Non-Concentrated Prep:
External Academic: $920
UNC Investigator: $600
Non-Concentrated Lentivirus Preps (100ml, 10E6 iu/ml)
Vectors in this category are suitable for in vitro studies. A total of 100ml vector prep is available in aliquots of either 5, 10 or 25ml (in DMEM 10% FCS). RCR assays are not mandatory for these vector preps. Approximate turnaround time is 2-3 weeks.
Prices per Non-Concentrated Prep:
External Academic: $750
UNC Investigator: $500
Corporate: $1,000
Specific Premade High Titer Concentrated/Purified Lentiviral Vectors (10ul)
10ul aliquots of Dr. Ed Boyden's lentivirus constructs are available, as well as CMV-GFP.
- CMV-GFP
- CamKII-ChR2-GFP
- CamKII-Arch-GFP
- CamKII-ArchT-GF
Prices per 10ul aliquot:
External Academic: $260
UNC Investigator: $170
* RCR assay is mandatory for concentrated preps, and optional ($50) for non-concentrated preps. |
