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UNC Vector Core Services

6101 Quadrangle Dr, Ste 200 

Chapel Hill, NC 27517

ph: 919-537-3100

fax: 919-537-3094

 

How to order:

The Vector Core now has online ordering! Please click here to place your order. 

PLEASE NOTE:  Faxing/emailing a PO does not place the order, it must be entered in our online system before we can process.  Thanks! 

If you have any questions regarding the ordering or billing process, please contact Lori Nisi. For questions regarding vector availability or vector production, please contact Xinghua Zeng via email or phone at 919.537.3100.

How to pay:

External customers:  We accept POs and Visa/Mastercard. 

  • PO payment - Please choose PO upon checkout and provide a billing contact where you would like the invoice sent.

  • Visa/MC - Please indicate you would like to pay with a card upon checkout and provide a contact for the card.  You will receive an email with a secure link to enter your credit card information after your order ships.  We do not charge until your order has been shipped.

  • Shipping Fee:  Vectors are shipped via Fedex.  Shipping charges are $40 per domestic order, $150 per international with the exception of Australia/New Zealand (~$1200, we obtain quote or can use your World Courier account).

UNC customers:  Please provide a funding account number.

Available Services:

*As of April 2014, we are no longer providing adenovirus services.  We recommend the Baylor College of Medicine facility.

* Please click here for vector storage and handling information

 

 

AAV Custom Vector Production (serotypes 1-6, 2.5, 8, 9)

Our new AAV vector production method utilizes a triple transfection protocol in suspension HEK293 cells and is purified by column chromotography. This system is serum free, antibiotic free, generates less empty particles, has higher purity and is scalable to Wave bioreactors.  We offer custom vector production and aliquots of in-stock vectors.

Custom AAV Vector Production

For custom vector preps, please send at least 300ug of plasmid.  Approximate turnaround time is 2-3 weeks.  We guarantee a titer of 10E11, although average yield is usually ~10E12.  Yield is dependent on customer construct.  For large scale, preclinical or clinical AAV vector production, please contact David Dismuke

Prices per Custom Prep:

External Academic:  $2,200

UNC Investigator:  $1,500

Corporate:  $3,400

Please send plasmids via FedEx or UPS to:

UNC Vector Core

Attn:  Xinghua Zeng

6101 Quadrangle Dr.

Room 206

Chapel Hill, NC 27517

*Please include a copy of your online order confirmation and a Sma1 restriction map with your plasmid shipment.

 

 

In-Stock AAV Vectors - Reporter Genes, 100ul Aliquots

Prices per 100ul aliquot:

External Academic: $220

UNC Investigator:  $150

Corporate:  $340

The following viruses are available in AAV1, 2, 2.5, 5, 6, 8 & 9:

  • GFP (CMV and CBA promoters)
  • Self-complementary GFP (CMV, CBA, CBh promoters)
  • AAV-CMV-Luciferase
  • AAV-CMV-lacZ

The following viruses are available in AAV2 & AAV5:

  • AAV-CMV-Cre-GFP
  • AAV-pkg-Cre

The following viruses are available in AAV8:

  • AAV-CaMKIIa-GFP-Cre
  • AAV-CaMKIIa-mCherry-Cre
  • AAV-GFAP-GFP-Cre
  • AAV-GFAP-mCherry-Cre
  • AAV-hSyn-GFP-Cre
  • AAV-hSyn-mCherry-Cre

 

 

In-Stock AAV Vectors - Dr. Karl Deisseroth, 100ul Aliquots

We are pleased to stock optogenetic constructs from Dr. Karl Deisseroth at Stanford UniversityFor first time orders of these viruses, Laboratory PIs please contact Dr. Karl Deisseroth and Charu Ramakrishnan for an MTA. Please upload a copy of your MTA when placing your online order.

The following constructs are available in AAV2 and AAV5.  For other serotypes, please place an order for a custom prep.

Optical Excitation:  ChR2

  • AAV-CaMKIIa-hChR2(H134R)-EYFP 
  • AAV-CaMKIIa-hChR2(H134R)-mCherry 
  • AAV-hSyn-hChR2(H134R)-EYFP 
  • AAV-hSyn-hChR2(H134R)-mCherry 
 

Cre-inducible AAV: DIO-AAV

  • AAV-EF1a-DIO-hChR2(H134R)-EYFP 
  • AAV-EF1a-DIO-hChR2(H134R)-mCherry 

 

Third-generation Optogenetic Inhibition: eNpHR3.0

  • AAV-EF1a-DIO-eNpHR3.0-EYFP 
  • AAV-EF1a-DIO-eNpHR3.0-mCherry 
  • AAV-CaMKIIa-eNpHR3.0-EYFP 
  • AAV-CaMKIIa-eNpHR3.0-mCherry 
  • AAV-hSyn-eNpHR3.0-EYFP 
  • AAV-hSyn-eNpHR3.0-mCherry 
  • AAV-hThy1-eNpHR3.0-EYFP 

 

Red-shifted Optical Excitation: C1V1 Variants

  • AAV-CaMKIIa-C1V1(E122T/E162T)-TS-EYFP 
  • AAV-CaMKIIa-C1V1(E122T/E162T)-TS-mCherry 
  • AAV-EF1a-DIO-C1V1(E122T/E162T)-TS-EYFP 
  • AAV-EF1a-DIO-C1V1(E122T/E162T)-TS-mCherry
  • AAV-CaMKIIa-C1V1(E162T)-p2A-EYFP

 

Stabilized Step Function Opsins: SSFOs

  • AAV-CaMKIIa-hChR2(C128S/D156A)-EYFP
  • AAV-CaMKIIa-hChR2(C128S/D156A)-mCherry 
  • AAV-EF1a-DIO-hChR2(C128S/D156A)-EYFP 
  • AAV-EF1a-DIO-hChR2(C128S/D156A)-mCherry 

 

Second-generation Ultrafast Optogenetic Control

  • AAV-CaMKIIa-hChR2(E123T/T159C)-EYFP 
  • AAV-CaMKIIa-hChR2(E123T/T159C)-mCherry 
  • AAV-CaMKII-hChR2(E123A)-EYFP 
  • AAV-CaMKII-hChR2(E123A)-mCherry 
  • AAV-EF1a-DIO-hChR2(E123T/T159C)-EYFP 
  • AAV-EF1a-DIO-hChR2(E123T/T159C)-mCherry 
  • AAV-EF1a-DIO-ChR2(T159C)-EYFP 
  • AAV-EF1a-DIO-ChR2(T159C)-mCherry 
  • AAV-EF1a-DIO-ChR2(E123A)-EYFP 
  • AAV-EF1a-DIO-ChR2(E123A)-mCherry 
  • AAV-hSyn-ChR2(E123A)-EYFP 

 

Third-generation Optogenetic Inhibition: Arch 3.0 & ArchT 3.0

  • AAV-CaMKIIa-eArch3.0-EYFP 
  • AAV-hSyn-eArch3.0-EYFP 
  • AAV-EF1a-DIO-eArch3.0-EYFP 
  • AAV-CaMKIIa-eArchT3.0-EYFP

Transsynaptic Tracers: WGA-Cre

  • AAV-EF1a-mCherry-IRES-WGA-Cre 

 

Ultrafast Optogenetic Control: ChETA

  • AAV-EF1a-DIO-ChETA-EYFP 

Cell Filling Variants

  • AAV-CaMKII-hChR2(T159C)-p2A-EYFP-WPRE
  • AAV-CaMKII-hChR2(T159C)-p2A-mCherry-WPRE
  • AAV-CaMKII-hChR2(E123T/T159C)-p2A-EYFP-WPRE
  • AAV-CaMKII-hChR2(E123T/T159C)-p2A-mCherry-WPRE
  • AAV-CaMKIIa-C1V1(E162T)-TS-p2A-EYFP-WPRE
  • AAV-CaMKIIa-C1V1(E162T)-TS-p2A-mCherry-WPRE
  • AAV-CaMKIIa-C1V1(t/t)-TS-p2A-EYFP-WPRE
  • AAV-CaMKIIa-C1V1(t/t)-TS-p2A-mCherry-WPRE
  • AAV-EF1a-DIO-hChR2(E123T/T159C)-p2A-EYFP-WPRE
  • AAV-EF1a-DIO-hChR2(E123T/T159C)-p2A-mCherry-WPRE
  • AAV-EF1a-DIO-C1V1(E162T)-TS-p2A-EYFP-WPRE
  • AAV-EF1a-DIO-C1V1(E162T)-TS-p2A-mCherry-WPRE
  • AAV-EF1a-DIO-C1V1(t/t)-TS-p2A-EYFP-WPRE
  • AAV-EF1a-DIO-C1V1(t/t)-TS-p2A-mCherry-WPRE

 

Control Fluorophores

  • AAV-CaMKIIa-EYFP
  • AAV-CaMKIIa-mCherry
  • AAV-EF1a-DIO-EYFP
  • AAV-EF1a-DIO-mCherry
  • AAV-hSyn-EYFP 
  • AAV-hSyn-mCherry 

 

The following constructs are available in AAV1:

  • AAV-GFAP-hChR2(H134R)-EYFP
  • AAV-GFAP-hChR2(H134R)-mCherry

 


In-Stock AAV Vectors - Dr. Ed Boyden, 100ul Aliquots

We are pleased to stock optogenetic constructs from Dr. Ed Boyden at MIT.  The following constructs are available in serotypes 1, 2, 5, 8 & 9.  For other serotypes, please place an order for a custom prep.  No MTA is required. 

Neural Silencers

  • AAV-CAG-ArchT-GFP
  • AAV-CAG-FLEX-ArchT-GFP

Neural Activators

  • AAV-CAG-ChR2-GFP

Control Vectors

  • AAV-CAG-FLEX-GFP
  • AAV-CAG-GFP
  • AAV-CAG-FLEX-tdTomato
  • AAV-CAG-tdTomato

The following constructs are available in serotypes 2 & 5:

Neural Silencers

  • AAV-CAG-FLEX-Arch-GFP
  • AAV-CAG-FLEX-ArchT-tdTomato
  • AAV-CAG-Arch-GFP
  • AAV-CAG-ArchT-tdTomato
  • AAV-CaMKII-ArchT-GFP

Update Feb 2014: We will be stocking Dr. Boyden's Chronos, Chrimson and Jaws constructs.  As soon as they are available, they will be added to the online ordering website.

 

 

In-Stock AAV Vectors - Dr. Bryan Roth, 100ul Aliquots

We are pleased to stock chemogenetic constructs from Dr. Bryan Roth at UNC.  The following constructs are available in AAV2, 5 & 8.  For other serotypes, please place an order for a custom prep.  No MTA is required.

Cre Dependent DREADDs

  • AAV-hSyn-DIO-EGFP
  • AAV-hSyn-DIO-hM3D(Gq)-mCherry
  • AAV-hSyn-DIO-hM4D(Gi)-mCherry
  • AAV-hSyn-DIO-rM3D(Gs)-mCherry
  • AAV-hSyn-DIO-mCherry

Non-Cre Dependent DREADDs

  • AAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine
  • AAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine
  • AAV-hSyn-EGFP
  • AAV-CaMKIIa-HA-hM3D(Gq)-IRES-mCitrine
  • AAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine
  • AAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine
  • AAV-CaMKIIa-EGFP
  • AAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine
  • AAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine
  • AAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine
  • AAV-GFAP-EGFP

The following constructs are currently available in AAV8:

Non-Cre Dependent DREADDs

  • AAV-hSyn-hM3D(Gq)-mCherry
  • AAV-hSyn-hM4D(Gi)-mCherry
  • AAV-CaMKIIa-hM3D(Gq)-mCherry
  • AAV-CaMKIIa-hM4D(Gi)-mCherry
  • AAV-GFAP-hM3D(Gq)-mCherry
  • AAV-GFAP-hM4D(Gi)-mCherry

 

 

In-Stock AAV Vectors - Dr. Naoshige Uchida, 100ul Aliquots

We are pleased to offer constructs from Dr. Naoshige Uchida at Harvard.The following constructs are available in AAV1, 5 & 8.  For other serotypes, please place an order for a custom prep.  No MTA is required.

  • AAV-CA-FLEX-RG
  • AAV-EF1a-FLEX-TVAmCherry

 

 

In-Stock AAV Vectors - Dr. Nirao Shah, 100ul Aliquots

We are pleased to offer constructs from Dr. Nirao Shah at The University of San Francisco. The following construct is currently available in AAV1 and AAV2. We also plan to stock AAV5.  For other serotypes, please place an order for a custom prep.  No MTA is required.

  • AAV-flex-taCasp3-TEVp

 

 

Lentivirus Vectors     Lentivirus MSDS

Recombinant lentiviral vectors are produced in 293T cells by transfection and can be purified/concentrated using sucrose gradients or by size exclusion.  Titers of physical vector particles are determined by p24 gag ELISA.  We translate 1 ng of p24 to 10E4 IU (for most vectors this is a significant underestimation of the actual vector titer).  Safety assays for replication competant retroviruses (RCR) include marker rescue assay and gag and tat transfer assays.  For information on lentivirus production, please contact Dr. Tal Kafri at 919-843-7365.

High Titer Concentrated/Purified Lentivirus Preps (100ul, >10E9 iu/ml)

For in vivo gene delivery studies, the UNC Vector Core recommends using high titer concentrated/purified vectors.  Approximate turnaround time is 6-7 weeks (this includes mandatory safety assays to rule out the emergence of replication competent retrovirus).  Please send at least 350ug of plasmid concentrated at 0.5 - 1.5mg/ml extracted by either Midi/Maxi prep columns or by cesium gradient. Alternatively, plasmid DNA can be prepared by the Vector Core for $250.

Please send plasmids to:

Dr. Tal Kafri

UNC Gene Therapy Center

5019 Thurston Bowles

Chapel Hill, NC 27599

Prices per Concentrated Prep (*includes RCR assay):

External Academic: $2,350

UNC Investigator: $1,550

Corporate:  $3,050

High Titer Non-Purified Lentivirus Custom Prep (100ul, 10E8-5x10E9 IU/ml)

Vectors in this category are suitable for in vitro studies which require gene delivery to relatively non-permissive target cells.  Typical vector titers range between 10E9 - 5x10E9 IU/ml (determined by the nature of the vector cassette).  The vectors are concentrated by size exclusion (RCR assays are not mandatory).  A total prep of 100ul can be provided in aliquots of either 10, 20 or 100ul.  Approximate turnaround time is 2-3 weeks.

Prices per Non-Concentrated Prep:

External Academic:  $920

UNC Investigator:  $600

 

Non-Concentrated Lentivirus Preps (100ml, 10E6 iu/ml)

Vectors in this category are suitable for in vitro studies.  A total of 100ml vector prep is available in aliquots of either 5, 10 or 25ml (in DMEM 10% FCS).  RCR assays are not mandatory for these vector preps.  Approximate turnaround time is 2-3 weeks.

Prices per Non-Concentrated Prep:

External Academic:  $750

UNC Investigator:  $500

Corporate:  $1,000

Specific Premade High Titer Concentrated/Purified Lentiviral Vectors (10ul)

10ul aliquots of Dr. Ed Boyden's lentivirus constructs are available,  as well as CMV-GFP. 


  • CMV-GFP
  • CamKII-ChR2-GFP
  • CamKII-Arch-GFP
  • CamKII-ArchT-GF

Prices per 10ul aliquot:

External Academic: $260

UNC Investigator: $170

* RCR assay is mandatory for concentrated preps, and optional ($50) for non-concentrated preps. 

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