Vector Core Services
The UNC Vector Core has relocated to a new facility. Operations will be down for approximately 1 week, so please expect delays. Thanks so much for your patience and understanding during this time. Our new address and contact info are listed below.
The lentiviral lab will not be moving, and will remain at the address listed under lentivirus ordering instructions.
How to order:
For
your convenience, the UNC Vector Core Order Form is available online.
Please email completed form to Lori Nisi. *Please note this is a change to the ordering process.
If you have any questions regarding the ordering or billing process, please contact Lori Nisi. For questions regarding vector availability or vector production, please contact Xinghua Zeng via email or phone at 919.537.3100.
*Please note: The Vector Core has relocated to:
6101 Quadrangle Dr..
Room 206
Chapel Hill, NC 27517
Ph: 919.537.3100
Available Services:
* Please click here for vector storage and handling information
* Please click here for our in-stock virus inventory
How to pay:
External customers: We accept POs and Visa/Mastercard.
-
PO payment - Please write PO# on order form. An invoice will be sent to the accounting contact after order is shipped.
-
Visa/MC - Please indicate you would like to pay with a card on the order form and provide a contact for the card. We will contact you for card details after the order is shipped. We do not charge until your order has been shipped.
- Shipping Fee: Vectors are shipped via Fedex. Shipping charges are $40 per domestic order, $150 per international.
UNC customers: Please provide a funding account number.
AAV Vector Production (serotypes 1-6, 2.5, 8, 9) AAV MSDS
Our new AAV vector production method utilizes a triple transfection protocol in suspension HEK293 cells and is purified by column chromotography. This system is serum free, antibiotic free, generates less empty particles, has higher purity and is scalable to Wave bioreactors. We offer custom vector production and aliquots of in-stock vectors.
Custom AAV Vector Production
For custom vector preps, please send at least 300ug of plasmid. Approximate turnaround time is 2-3 weeks. We guarantee a titer of 10E11, although average yield is usually ~10E12. Yield is dependent on customer construct.
Prices per Custom Prep:
External Academic: $2,200
UNC Investigator: $1,500
Corporate: $3,400
Please send plasmids via FedEx or UPS to:
UNC Vector Core
6101 Quadrangle Dr.
Room 206
Chapel Hill, NC 27517
*Please include a copy of your order form and a Sma1 restriction map with your plasmid shipment.
In-Stock AAV Vectors, 100ul Aliquots
Prices per 100ul aliquot:
External Academic: $220
UNC Investigator: $150
Corporate: $340
The following viruses are available in AAV1, 2, 2.5, 5, 6, 8 & 9:
- GFP (CMV and CBA promoters)
- Self-complementary GFP (CMV, CBA, CBh promoters)
- AAV-CMV-Luciferase
- AAV-CMV-lacZ
In addition to our reporter gene inventory, we are pleased to stock optogenetic constructs from Dr. Karl Deisseroth at Stanford University, Dr. Ed Boyden at MIT and Dr. Bryan Roth at UNC.
Dr. Karl Deisseroth Constructs
For first time orders of these viruses, Laboratory PIs please contact Dr. Karl Deisseroth and Charu Ramakrishnan for an MTA. Please submit a copy of your MTA along with your Vector Core order form each time you place an order for these vectors.
The following constructs are available in AAV2 and AAV5. For other serotypes, please place an order for a custom prep.
- AAV-EF1a-DIO-EYFP
- AAV-EF1a-DIO-mCherry
- AAV-EF1a-DIO-hChR2(H134R)-EYFP
- AAV-EF1a-DIO-hChR2(H134R)-mCherry
- AAV-EF1a-DIO-eNpHR 3.0-EYFP
- AAV-EF1a-DIO-ChETA-EYFP
- AAV-CaMKIIa-EYFP
- AAV-CaMKIIa-mCherry
- AAV-CaMKIIa-hChR2(H134R)-EYFP
- AAV-CaMKIIa-hChR2(H134R)-mCherry
- AAV-CaMKIIa-eNpHR 3.0-EYFP
- AAV-hSyn-hChR2(H134R)-EYFP
- AAV-hSyn-hChR2(H134R)-mCherry
- AAV-hSyn-eNpHR 3.0-EYFP
- AAV-CaMKIIa-C1V1(E122T/E162T)-TS-EYFP
- AAV-CaMKIIa-C1V1(E122T/E162T)-TS-mCherry
- AAV-EF1a-DIO-C1V1(E122T/E162T)-TS-EYFP
- AAV-EF1a-DIO-C1V1(E122T/E162T)-TS-mCherry
- AAV-CaMKIIa-hChR2(C128S/D156A)-EYFP
- AAV-CaMKIIa-hChR2(C128S/D156A)-mCherry
- AAV-EF1a-DIO-hChR2(C128S/D156A)-EYFP
- AAV-EF1a-DIO-hChR2(C128S/D156A)-mCherry
- AAV-CaMKIIa-hChR2(E123T/T159C)-EYFP
- AAV-CaMKIIa-hChR2(E123T/T159C)-mCherry
- AAV-CaMKIIa-hChR2(E123A)-EYFP-WPRE
- AAV-CaMKIIa-hChR2(E123A)-mCherry-WPRE
- AAV-EF1a-DIO-hChR2(E123T/T159C)-EYFP
- AAV-EF1a-DIO-hChR2(E123T/T159C)-mCherry
- AAV-EF1a-DIO-ChR2(E123A)-EYFP-WPRE
- AAV-EF1a-DIO-ChR2(E123A)-mCherry-WPRE
- AAV-EF1a-DIO-eNpHR 3.0-mCherry-WPRE
- AAV-CaMKIIa-eNpHR 3.0-mCherry-WPRE
- AAV-hSyn-eNpHR 3.0-mCherry-WPRE
- AAV-hSyn-ChR2(E123A)-EYFP-WPRE
- AAV-hSyn-EYFP
- AAV-EF1a-mCherry-IRES-WGA-Cre
Dr. Ed Boyden Constructs
The following constructs are available in serotypes 1, 2, 5, 8 and 9. For other serotypes, please place an order for a custom prep.
- AAV-CAG-ArchT-GFP
- AAV-FLEX-ArchT-GFP
- AAV-CAG-ChR2-GFP
Dr. Bryan Roth Constructs
The following constructs are available in AAV2 and AAV5. For other serotypes, please place an order for a custom prep.
Cre Dependent DREADDs
- AAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine
- AAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine
- AAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine
- AAV-hSyn-DIO-EGFP
- AAV-hSyn-DIO-hM3D(Gq)-mCherry
- AAV-hSyn-DIO-hM4D(Gi)-mCherry
- AAV-hSyn-DIO-rM3D(Gs)-mCherry
- AAV-hSyn-DIO-mCherry
- AAV-EF1a-DIO-hM3D(Gq)-mCherry
- AAV-EF1a-DIO-hM4D(Gi)-mCherry
- AAV-EF1a-DIO-mCherry
Non-Cre Dependent DREADDs
- AAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine
- AAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine
- AAV-hSyn-EGFP
- AAV-CaMKIIa-HA-hM3D(Gq)-IRES-mCitrine
- AAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine
- AAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine
- AAV-CaMKIIa-EGFP
- AAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine
- AAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine
- AAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine
- AAV-GFAP-EGFP
For large scale, preclinical or clinical AAV vector production, please contact David Dismuke.
Adenovirus Amplification Prep Adenovirus MSDS
Recombinant Ad vectors are amplified in 293 cells and purified with 2 consecutive CsCl gradients. Vectors are titered by infecting target cells and detecting infected cells with antibody staining. Preps have a guaranteed titer of 10E12 total particles and approximate turnaround time is 3-4 weeks.
Prices per 1ml Prep:
External Academic: $900
UNC Investigator: $600
Corporate: $1400
In-Stock Adenovirus Vectors, 100ul Aliquots
Prices per 100ul aliquot:
External Academic: $90
UNC Investigator: $60
Corporate: $140
Generation of Adenovirus Recombinants Adenovirus MSDS
The AdEasy system as described by Vogelstein's laboratory is used to incorporate new genes into Ad vectors. Cell lysates are then provided to the investigator for testing prior to amplification of the vector. Approximate turnaround time is 6 weeks.
Prices:
External Acadmic: $1,400
UNC Investigator: $900
Corporate: $2,200
Lentivirus Vectors Lentivirus MSDS
Recombinant lentiviral vectors are produced in 293T cells by transfection and are purified/concentrated using sucrose gradients. Titers of physical vector particles are determined by p24 gag ELISA. Safety assays for replication competant retroviruses (RCR) include marker rescue assay and gag and tat transfer assays. For information on lentivirus production, please contact Dr. Tal Kafri at 919-843-7365.
Concentrated Lentivirus Preps (100ul, 10E9 iu/ml)
Approximate turnaround time is 6-7 weeks. Please send at least 750ug of plasmid concentrated at 1mg/ml. Cesium plasmid preps are recommended, and can be prepared by the Vector Core.
Please send plasmids to:
Dr. Tal Kafri
UNC Gene Therapy Center
5019 Thurston Bowles
Chapel Hill, NC 27599
Prices per Concentrated Prep (*includes RCR assay):
External Academic: $2,350
UNC Investigator: $1,550
Corporate: $3,050
20ul aliquots of Dr. Ed Boyden's lentivirus constructs are available, please contact Dr. Tal Kafri for construct and availability information.
Non-Concentrated Lentivirus Preps (100ml, 10E6 iu/ml)
Approximate turnaround time is 3-4 weeks.
Prices per Non-Concentrated Prep:
External Academic: $750
UNC Investigator: $500
Corporate: $1,000
* RCR assay is mandatory for concentrated preps, and optional ($50) for non-concentrated preps.
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