Vector Core Services

For your convenience, the Vector Core Order Form is available on-line.  Please fax completed form to 919.966.9218.

Click here for a list of in-stock vectors.  Click here for information on the storage and handling of vector products.  If you have any questions, please contact the Vector Core at 919.962.1221.

If you are sending plasmids, please send them to:

UNC Vector Core

7017 Neurosciences Hospital

101 Manning Dr.

Chapel Hill, NC 27599

AAV vector production (serotypes 1-6)      AAV MSDS
AAV vectors are generated using a triple transfection protocol and purified using a sequential process of nuclei isolation, density gradient centrifugation and heparin sulfate affinity column chromatography. 

Vectors are titered by dot-blot and guaranteed to have at least 10E11 total particles.  Approximate turnaround time for preps: 3-4 weeks.   If you are sending plasmids, please send at least 200 ug.
Academic Price : $2200 per prep

                                $220 per 100 µl aliquot of in-stock vector

Corporate Price: $3400 per prep

                               $340 per 100 µl aliquot of in-stock vector

*For large corporate orders on all vectors, please contact Jeff Beecham for price negotiation

Adenovirus vector production      Adenovirus MSDS
Recombinant Ad vectors are amplified in 293 cells and purified with 2 consecutive CsCl gradients.  Vectors are titered by infecting target cells and detecting infected cells with antibody staining.  Preps have a guaranteed titer of 10E12 total particles, and approximate turnaround time is 3-4 weeks.

Academic Price: $900 per 1 ml prep

                              $90 per 100 µl aliquot of in-stock vector

Corporate Price: $1400 per 1 ml prep

                       $140 per 100 µl aliquot of in-stock vector

Generation of adenovirus recombinants      Adenovirus MSDS
The AdEasy system as described by Vogelstein's laboratory is used to incorporate new genes into Ad vectors.  Cell lysates are then provided to the investigator for testing prior to amplification of the vector.   Approximate turnaround time: 6 weeks.

Academic Price: $1400 per construct

Corporate Price: $2200 per construct

Lentivirus vectors      Lentivirus MSDS
Recombinant lentiviral vecors are produced in 293T cells by transfection and are purified/concentrated using sucrose gradients. Titers of physical vector particles are determined by p24 gag.  ELISA. Safety assays for replication competent retroviruses (RCRs) include marker rescue assay and gag and tat  transfer assays. 

The Vector Core offers concentrated preps (100 µl, 10E9 iu/ml) and non-concentrated preps (100ml, 10E6 iu/ml).  Approximate turnaround time for concentrated preps: 6-7 weeks; non-concentrated preps: 3-4 weeks.

If you are sending plasmids, please send at least 750 ug concentrated at 1mg/ml.  Cesium plasmid preps are recommended, and can be prepared by the Vector Core for an additional $250.
Academic Price: $1500 per concentrated prep

                              $500 per non-concentrated prep

Corporate Price: $2300 per concentrated prep

                              $750 per non-concentrated prep

                              *For Replication Competant Retrovirus (RCR) Assay, please add $50

For large scale preclinical and clinical grade vectors, please contact Jeff Beecham.