Material Transfer Agreement
This page has been created to provide the outside research community with convenient access to our vector sequences and our materials transfer agreement. We have included the sequences and references to the most requested plasmid vectors created in our laboratory.
We are now distributing our materials through the National Gene Vector Biorepository (NGVB). To request an MTA, please visit the NGVB website.
If you would like to obtain our other biological materials not available through the NGVB, please email Jude Samulski .
Maps and Sequences
pSub201 was first described in the Journal of Virology (volume 61, number 10, pp.3096-3101) October 1987 by Samulski et al. This vector contains all of the Adeno-Associated Virus (AAV-2) type 2 wild-type coding regions and cis acting terminal repeats cloned into a plasmid backbone. This vector is ideal for cloning, being that it was engineered in such a way that restriction digest with Xba I allows one to remove the AAV coding region while leaving the AAV terminal repeats intact in the plasmid backbone. This is very important because the terminal repeats are the only cis acting sequences required for recombinant virus production.
pAAV/Ad serves as the trans-complimenting vector in recombinant AAV production. This vector was first described in the Journal of Virology (volume 63, number 9, pp. 3822-3828) September 1989. This vector This construct contains the AAV rep and cap coding regions with adenovirus terminal repeats at each end. The vector backbone for this vector is pEMBL.
pAd8 serves as the trans-complimenting vector in recombinant AAV production. This vector contains the same insert as pAAV/Ad (AAV rep and cap coding regions with an Adenovirus terminal repeat at each end) cloned into the pBR322 vector backbone(w/tet gene deleted).