Material Transfer Agreement

This page has been created in order to provide the outside research community with convenient access to our vector sequences and our materials transfer agreement. We have included the sequences and references to the most requested plasmid vectors created in our laboratory. In order to obtain these vectors, we ask that you fill out a material transfer agreement and follow the instructions therein.

• For sub201, pXX2, pXX6-80, XX2-lacZ, DX11, XX2-GFP3, ACG or AB11 plasmids, please fill out MTA form 1.

• For pvp2gluc2, pvp2gfp2, pXR1, pXR2, pXR3, pXR4, pXR5, pXR6, pXR1.1, pXR1.2, pXR1.3, pXR1.4, pXR1.5, pXR1.6, pAAV6.1, pAAV6.2, pAAV6.3, pAAV6.4, pAAV6.5, pAAV6.6, AAV2.5 and ScAAV2-CMV-GFP plasmids, please fill out MTA form 2.

Please complete the MTA form, have your institution sign and mail to:

UNC Chapel Hill

Office of Technology Development

CB# 4105, 308 Bynum Hall

Chapel Hill, NC 27599-4105

Upon full execution of the MTA, the Vector Core will contact you to arrange shipment of requested materials. 

If you would like to obtain our other biological materials that are not listed below, please email Jude Samulski .

Maps and Sequences

pSub201 was first described in the Journal of Virology (volume 61, number 10, pp.3096-3101) October 1987 by Samulski et al. This vector contains all of the Adeno-Associated Virus (AAV-2) type 2 wild-type coding regions and cis acting terminal repeats cloned into a plasmid backbone. This vector is ideal for cloning, being that it was engineered in such a way that restriction digest with Xba I allows one to remove the AAV coding region while leaving the AAV terminal repeats intact in the plasmid backbone. This is very important because the terminal repeats are the only cis acting sequences required for recombinant virus production.

pAAV/Ad serves as the trans-complimenting vector in recombinant AAV production. This vector was first described in the Journal of Virology (volume 63, number 9, pp. 3822-3828) September 1989. This vector This construct contains the AAV rep and cap coding regions with adenovirus terminal repeats at each end. The vector backbone for this vector is pEMBL.

pAd8 serves as the trans-complimenting vector in recombinant AAV production. This vector contains the same insert as pAAV/Ad (AAV rep and cap coding regions with an Adenovirus terminal repeat at each end) cloned into the pBR322 vector backbone(w/tet gene deleted).